Download An introduction to metabolic and cellular engineering by S Cortassa; et al PDF

By S Cortassa; et al

ISBN-10: 981238877X

ISBN-13: 9789812388773

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Extra resources for An introduction to metabolic and cellular engineering

Sample text

E. MFA and MCA (see Chapter 4). General Formulation of Mass Balance The first law of thermodynamics states that during any chemical or physical change (subatomic reactions are excluded), there is no modification of the total amount of matter participating. 1) where Minput stands for the input of matter to the system; Mprod is the matter produced during the process; Moutput stands for the output of matter from the system; Mconsum is the matter consumed; Maccumul is the matter accumulated in the system during the process.

8). Introduction 37 enzymatic rates and metabolites concentration are entrained by the MTP polymeric status (Fig. 1A, B) whereas phase plane analysis shows that the flux through glycolysis coherently changes with the level of polymerized MTP (Fig. 1C) as reflected by augmenting or diminishing the glycolytic flux concomitant with an increase or decrease of polymer levels. , 2001; Aon and Cortassa, 2001, unpublished). Biochemically and thermodynamically, cellular metabolism may be represented as a set of catabolic and anabolic fluxes coupled to each other through energy-transducing events.

Introduction 23 allometric law (Rosen, 1967, 1970; Aon and Cortassa, 1997). In the case of balanced growth these two state variables may represent N, a population of microbes (biomass), and M, a population of macromolecules (protein, carbohydrate) synthesized in a constant proportion with the microbial biomass. Growth is balanced when the specific rate of change of all metabolic variables (concentration or total mass) is constant (see eq. , 1995): Hence the fact that several sub- and supra-cellular systems scale their functioning in space and time exponentially, suggests that: (i) a defined relationship exists between the whole (cell) and each of its constituents (macromolecules); (ii) processes happen over broad time scales, given by their relaxation times following perturbation (see below).

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